@article {2789,
	title = {Presence of rhodanese in the cytosolic fraction of the fruit bat (<i>Eidolon helvum</i>) liver},
	journal = {Journal of Biochemistry \& Molecular Biology},
	volume = {37},
	number = {3},
	year = {2004},
	month = {2004},
	pages = {275-281},
	abstract = {Rhodanese was isolated and purified from the cytosolic fraction of liver tissue homogenate of the fruit bat, <i>Eidolon helvum</i>, by using ammonium sulphate precipitation and CM-Sephadex C-50 ion exchange chromatography. The specific activity was increased 130-fold with a 53\% recovery. The Km values for KCN and Na2S2O3 as substrates were 135 {\textpm} 2.2 mM and 195 {\textpm} 0.7 mM, respectively. The apparent molecular weight was estimated by gel filtration on a Sephadex G-100 column to be 36,000 Da. The optimal activity was found at a high pH (pH 9.0) and the temperature optimum was 35{\textdegree}C. An Arrhenius plot of the heat stability data consisted of two linear segments with a break occurring at 35{\textdegree}C. The apparent activation energy values from these slopes were 11.5 kcal/mol and 76.6 kcal/ mol. Inhibition studies on the enzyme with a number of cations showed that Mg2+, Mn2+, Ca2+, and Co2+ did not affect the activity of the enzyme, but Hg2+ and Ba2+ inhibited the enzyme.},
	keywords = {biochemistry, Chiroptera, Eidolon helvum, Nigeria, Pteropodidae, West Africa},
	author = {Agboola, F. K. and Okonji, R. E.}
}
